1株分离自海水中芘降解菌的鉴定及其降解特性研究     

王静  邱金泉  张雨山  杨波  张晓青  苗英霞  张爱君  张辉 《海洋学研究》2011,29(1):49-55

以芘为唯一碳源,对采自于天津港石油污染区的海水和土壤样品进行富集培养,分离到1株芘降解菌,经显微形态观察、生理生化鉴定、16S rRNA基因序列的比对及系统发育进化的分析,确定该菌株为施氏假单孢菌Pseudomonas stutzeri,并采用室内培养方法,对其进行了芘降解性能的测定及降解途径的分析.结果表明,该菌株在...  相似文献   

Influence of Biological Treatment and Ultraviolet Disinfection System on Pseudomonas spp. Diversity in Wastewater as Assessed by Denaturing Gradient Gel Electrophoresis          下载免费PDF全文

Inès Mehri  Yousra Turki  Hanène Chérif  Amel Khessairi  Abdennasser Hassen  Maher Gtari 《洁净——土壤、空气、水》2014,42(5):578-585

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Management of the precious coral fishery in Taiwan: Progress and perspectives     

Chih-Shin Chen 《Marine Policy》2012,36(3):623-629

Precious corals have been commercially exploited because of their legendary and cultural importance for many centuries in the Mediterranean and for almost one century in the Northwest Pacific. Although the precious coral fishery in Taiwan dates back to the 1920s, relevant studies on biology and ecology of resources are relatively scarce. The management of the precious coral fishery in Taiwan was officially instituted in January 2009, when data on the catch and effort of the fishery were recorded. In this study, the catch-effort data of the fishery were examined to explore the temporal and spatial patterns in composition and abundance of Corallium and Paracorallium spp. around Taiwan. A total landing of 3233.0 and 2906.8 kg precious corals were recorded for 2009 and 2010, respectively. Less than 5% of those amounts were from live colonies. A high proportion of fossilized colonies in the catch reflect the non-selective nature of the fishing gear; however, the factors that cause colonies to collapse are not exclusive to the impact of the fishery. Momo coral was dominant in the production for both years, followed by Miss coral. The total production of precious corals correlated positively with fishing efforts, while a weak relationship was found between the production of live colonies and fishing efforts. The fishing efforts mainly aggregated in designated fishing ground A (DFG-A) for both years, while the production of live colonies mainly occurred in DFG-E. The monthly occurrence rate of live colonies decreased over the past 2 years. Although fluctuating significantly, the CPUE of dominant species exhibited an apparent decreasing pattern. High aggregation of the fishing efforts in specific regions and low numbers of live colonies in the production should be carefully taken into account when amending management regulations in the future. The establishment of marine protected areas, in addition to the strict controls that already apply, should be considered to not only sustain the population structure of the sessile animals but also their function in the marine ecosystem.  相似文献   

富含古罗糖醛酸的褐藻多糖生产菌株的构建          下载免费PDF全文

胡斌  韩峰  林育姿  宫倩红  于文功 《海洋科学》2008,32(1):14-18

为获得古罗糖醛酸(Guluronate)含量高的细菌胞外褐藻多糖,利用PCR从海洋细菌Pseudomonassp.QDA中克隆了其甘露糖醛酸C-5差向异构酶基因(algG),连接入质粒pMF 54Km,构建了重组表达载体pMF54 Km-algG。利用三亲接合法将pMF54 Km-algG转入菌株QDA中,获得algG过量表达重组菌株QDA-G1。H-NMR测定结果表明,QDA-G所产的褐藻多糖中β-D-甘露糖醛酸(M)与它的C-5差向异构体α-L-古罗糖醛酸(G)的比值为0.38,G的质量分数达到74.2%,比野生菌株QDA提高了26.4%。且重组菌株遗传稳定性良好,连续传代20代后,M/G的比值无明显变化。  相似文献   

Ex situ cultivation of Aplysinaaerophoba close to in situ conditions: ecological, biochemical and histological aspects     

Anne Klöppel  Martin Pfannkuchen  Annika Putz  Peter Proksch  & Franz Brümmer 《Marine Ecology》2008,29(2):259-272

Sponges provide the largest number of biologically active natural products known from the marine environment and continue to be a very well studied phylum of marine fauna. The Mediterranean sponge Aplysina aerophoba accumulates brominated isoxazoline alkaloids such as Aplysinamisin-1, Aerophobin-2, Isofistularin-3 and the biotransformation product Aeroplysinin-1, which possesses, for example, antibiotic and cytotoxic properties. Until now, it is still being discussed which organisms – the sponge itself or associated microorganisms – are responsible for metabolite production. For cultivating Aplysina individuals under ex situ conditions, we surveyed relevant ecological factors in situ and controlled them in our aquarium system. We maintained A. aerophoba for more than 9 months and analysed changes of metabolite content and composition, microbial association as well as morphology in situ and ex situ under different light exposure. Although sponges showed slight reduction during maintenance, ex situ cultivation similar to in situ conditions provides a promising method to keep sponges and obtain their bioactive metabolites.  相似文献   

不同生物量的乳酸杆菌对对虾养殖池3种常见优势微藻的影响   总被引：3，自引：0，他引：3  

王昊  李卓佳  李纯厚  曹煜成  杨莺莺 《台湾海峡》2009,28(2):223-227

在实验室条件下,分别把对虾养殖池常见优势种——微绿球藻（Nannochloropsis oculata）、隐藻（Cryptomonas erosa）和颤藻（Oscillatoria）与乳酸杆菌（Lactobacillusspp.）在对虾养殖水体中共同培养,研究不同生物量的乳酸杆菌对3种微藻的影响.实验分4组,乳酸杆菌分C1（10^3cells/cm^3）组、C2（10^4cells/cm^3）组、C3（10^5cells/cm^3）组、C4（10^6cells/cm^3）组四个浓度.结果表明：不同生物量的乳酸杆菌对不同微藻的影响有很大差异.C1（10^3cells/cm^3）乳酸杆菌对微绿球藻的生长没有明显的影响,对隐藻和颤藻生长有明显的抑制作用.C2（10^4cells/cm^3）乳酸杆菌对微绿球藻和隐藻生长有明显的促进作用,对颤藻生长有明显的抑制作用.C3（10^5cells/cm^3）乳酸杆菌对隐藻生长有明显的抑制作用,对颤藻的生长有明显的促进作用.C4（10^6cells/cm^3）乳酸杆菌对微绿球藻和颤藻生长有较明显的促进作用,对隐藻生长有微弱的抑制作用.无论是抑制作用还是促进作用,这种作用都随实验时间的延长而减弱.  相似文献   

危害我国红树林的团水虱的生物学特征          下载免费PDF全文

杨玉楠  MYAT Thiri  刘晶  王瑶 《应用海洋学学报》2018,37(2):211-217

近些年来,团水虱(Sphaeroma spp.)在红树林生态系统中的持续爆发已成为天然和人工红树林遭受破坏的主要原因之一,在我国海南、广东和广西都有不同程度的发生.目前国内有关红树林湿地团水虱的生物学特征研究较少,而明确团水虱的生物学特征是研发其防控技术的重要依据.本研究通过文献查阅和实地调查,总结了团水虱危害中国红树林的现状,团水虱的种类、分布和生活史,以及繁殖和聚集的季节、繁殖方式、营养物质偏好等,以期为团水虱的防控提供理论和技术方面的支撑.  相似文献   

产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究   总被引：2，自引：0，他引：2  

张金伟  曾润颖 《台湾海峡》2005,24(4):520-525

从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验．实验结果证明,在以0．5％可溶性淀粉为碳源,以0．5％豆饼粉为氮 源,无机盐:0．424％NH4H2PO4、0．075％K2HPO4、0．02％MgSO4、0．5％CaCO3,起始 pH值8．5,接种36h种龄的种子10％,20℃、250r／min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高．最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22．4U／cm3和30．9U／cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景．  相似文献   

假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因克隆与鉴定     

刘广发  张会永  谢金镇 《海洋与湖沼》2005,36(4):313-318

采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp．cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明，该结构基因长819bp，与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61．5％，氨基酸同源性为56．2％。该基因已输入GenBank数据库，收录号AF348165。将该基因转化大肠杆菌，受体菌在含NaCl 1．0mol／L的培养基中甘油含量升高2．9倍，最终菌浓度提高3．6倍。可见这是一个与生物耐盐性相关的主基因，以其转化、培育耐盐农作物的前景十分光明。  相似文献   

鳗鲡气单胞菌引起的烂尾病病原菌抑菌药物的研究   总被引：5，自引：0，他引：5  

樊海平  陈茂辉 《海洋湖沼通报》1995,(4):61-65

应用药敏纸片法研究了三株烂尾病病原菌（气单胞菌）对３１种药物及不同配合药物的敏感性，测定了４种药物及配合药物ＨＰ对三株菌的最低抑菌浓度（ＭＩＣ）及最低杀菌浓度（ＭＢＣ）。结果表明，配合药物ＨＰ具较强的抑菌能力。  相似文献